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Metabolic and Oxidative Stress Core Laboratory

Director: Sinha-Hikim, Indrani, Ph.D.

Summary:

Since its inception fifteen years ago, the Technology Core Laboratory has surpassed its goals in new assay development and assay refinement. It has provided top-quality services, been innovative in developing new assays, and has encouraged collaborations between molecular biologists and clinical investigators. This core laboratory has an outstanding record of accomplishments and has supported many high-impact, peer-reviewed publications.

Affiliations:

People:

Resources:

Services

  • Activity assays ( Material analysis service )

    "Activity assays utilize either a chemical change to determine the presence or amount of a particular molecule, or monitor the enzyme activity of an enzyme utilizing some novel read out. We will research various options available and design, synthesize and validate new activity methods."

  • Apoptosis/ survival pathways assay service ( Material analysis service )

    Phospho & total p38 MAP kinase
    Extracellular Regulated kinase
    (ERK), AKT, C-Jun-NH2-Terminal kinase (JNK) (ALL EIA / Multiplex)
    PARP (ELISA and W.B)
    Caspase 3, 7, 8, 9 (ELISA, Multiplex)/EIA
    Cytochrome C (ELISA)

  • Assay analysis consulting services ( Analysis service )

    "Full consulting services for all aspects of an assay, application of a wide array of new technologies, explaining the results, and helping in writing methodology for papers and reports for the assays done in our laboratory."

  • Cancer marker immunoassay service ( Material analysis service )

    PSA

  • Cardiovascular immunoassay service ( Material analysis service )

    C-reactive Protein

  • Competitive immunoassays ( Material analysis service )

    "We are experts in the development of super-fast and/or super-sensitive ELISA's and multiplexed assays."

  • DNA damage/repair assay service ( Service )

    Telomerase Activity (PCR, Northern blotting)
    Telomerase length (PCR, Northern blotting)
    DNA Damage (8-hydroxy-2Æ-deoxyguanosine) (ELISA)

  • HIV/AIDS assay service ( Material analysis service )

    Epinephrine
    Norepinephrine
    Neopterin
    Dopamine

  • Hormone immunoassay service ( Material analysis service )

    Adiponectin (RIA)
    Aldosterone (RIA)
    Corticosterone (RIA)
    Cortisol (RIA/EIA)
    Dehydroepiandrosterone (EIA)
    DHEA (RIA)
    DHT (Men only) (column separation followed by RIA)
    Estradiol (RIA/ELISA)
    FSH (Delfia, Fluorometric assay)
    Gastrin (RIA)
    Ghrelin (RIA)
    IGF 1 (RIA)
    Insulin (RIA)
    Interleukin 1, IJ 5, IL6 (ELISA)
    Leptin (IRMA)
    Lh (Delfia, Fluorometric assay)
    Progesterone (RIA)
    SHBG (Delfia, Fluorometric assay)

  • Inflammation marker immunoassay service ( Material analysis service )

    C-reactive Protein (ELISA)
    PAI 1 (ELISA)
    Interleukin 2/4 (ELISA)

  • Kidney function immunoassay service ( Material analysis service )

    Pyro phosphate

  • Metabolic profile assay service ( Material analysis service )

    Oxidized LDL (ELISA)
    Free-fatty acids
    Triglycerides

  • Oxidative stress immunoassay service ( Material analysis service )

    TNF alpha (RIA/EIA)
    GSH/GSSG ratio (Colorimetric Assay)
    Glutathione Peroxidase activity (ELISA)
    Lipid peroxidation (4-HNE) (ELISA)
    TNF ? (ELISA)
    Telomerase Length
    Telomerase activity
    DNA Damage (8-OHDG) (ELISA)

  • Sandwich immunoassays ( Material analysis service )

    "Selection of solid phase antibodies based upon performance criteria developed from your assay specifications. The readout can be HRP or AP colorimetric detection to fluorescent, chemiluminescent or bioluminescent ELISA's"


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Last updated: 2012-04-18T13:07:40.591-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016