Since its inception fifteen years ago, the Technology Core Laboratory has surpassed its goals in new assay development and assay refinement. It has provided top-quality services, been innovative in developing new assays, and has encouraged collaborations between molecular biologists and clinical investigators. This core laboratory has an outstanding record of accomplishments and has supported many high-impact, peer-reviewed publications.
"Activity assays utilize either a chemical change to determine the presence or amount of a particular molecule, or monitor the enzyme activity of an enzyme utilizing some novel read out. We will research various options available and design, synthesize and validate new activity methods."
Phospho & total p38 MAP kinase
Extracellular Regulated kinase
(ERK), AKT, C-Jun-NH2-Terminal kinase (JNK) (ALL EIA / Multiplex)
PARP (ELISA and W.B)
Caspase 3, 7, 8, 9 (ELISA, Multiplex)/EIA
Cytochrome C (ELISA)
"Full consulting services for all aspects of an assay, application of a wide array of new technologies, explaining the results, and helping in writing methodology for papers and reports for the assays done in our laboratory."
"We are experts in the development of super-fast and/or super-sensitive ELISA's and multiplexed assays."
Telomerase Activity (PCR, Northern blotting)
Telomerase length (PCR, Northern blotting)
DNA Damage (8-hydroxy-2Æ-deoxyguanosine) (ELISA)
DHT (Men only) (column separation followed by RIA)
FSH (Delfia, Fluorometric assay)
IGF 1 (RIA)
Interleukin 1, IJ 5, IL6 (ELISA)
Lh (Delfia, Fluorometric assay)
SHBG (Delfia, Fluorometric assay)
C-reactive Protein (ELISA)
PAI 1 (ELISA)
Interleukin 2/4 (ELISA)
Oxidized LDL (ELISA)
TNF alpha (RIA/EIA)
GSH/GSSG ratio (Colorimetric Assay)
Glutathione Peroxidase activity (ELISA)
Lipid peroxidation (4-HNE) (ELISA)
TNF ? (ELISA)
DNA Damage (8-OHDG) (ELISA)
"Selection of solid phase antibodies based upon performance criteria developed from your assay specifications. The readout can be HRP or AP colorimetric detection to fluorescent, chemiluminescent or bioluminescent ELISA's"